On 3 Oct 1996, Dr. Michael Ziman wrote:
>> I was wondering, why one has to use minimal plates for 5-FOA. Can't I just
> use YPD?
>> Any comments would be appreciated!
The use of 5-FOA is to select against the functional URA3 gene.
If you have a strain with this gene functional, naturally, it will grow i
YPD plates. You must to give 5-FOA - an analog of pyrimidine - for this
type of "negative seklection".