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Immunofluorescence Backround

Ricardo Azpiroz azpiroz at U.ARIZONA.EDU
Sat Oct 5 14:52:40 EST 1996

Check out Azpiroz, R. and R. Butow (1995), Methods Enzymol. 260, p.? There
is a simple method to preclear antibodies with protein extracts adsorbed
to nitrocellulose. It works like a charm, even on really dirty antisera.
Affinity jpurification is nice, but it is laborious and kills the antibody
within weeks after purification.


Azpiroz, R.

On 4 Oct 1996 GMEACHAM at bmg.bhs.uab.edu wrote:

To all, 
	I am doing indirect immunofluorescence on S.cerevisiae YPH499. I am 
having a problem with high backround i.e. the preimmune control lights 
up like a Christmas tree. We are about to affinity purify the Ab. What 
fixatives or levels of blocking has anyone had success in reducing 
backround? Our Ab looks pretty good on a western. Thanks in advance.

Geoff Meacham
UAB Dept. of Cell Biology

Ricardo Azpiroz
Department of Biochemistry
Life Sciences South
University of Arizona
Tucson AZ 85721-0106

azpiroz at U.Arizona.EDU

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