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Colony PCR

Duncan Greig duncan.greig at st-cross.ox.ac.uk
Thu Oct 3 09:05:29 EST 1996


I am trying to PCR up a 2kb fragment, and am having difficulty getting it
to appear using colony PCR I have had good results with the primers after
making DNA, but it would be convinient to be able to use colonies.

Does anyone have a protocol that has produced such large fragments reliably?

thanks.

Aidan Weatherill.

please reply here or E-mail me at
aweatherill%immads2.jr2 at ox.ac.uk

Thanks



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