Dear Leibowitz:
The references below bear on okadaic acid and also sphingolipids and
ceramide. Fisbein tests okadaic on yeast.
Cuvillier, O. et al. (1996). Suppression of ceramide-mediated cell death
by
sphingosin-1-phosphate. Nature, 381(27 jun), 800-803.
Fishbein, J. D., Dobrowski, R. T., Bielawska, A., Garret, S., & Hannun,
Y.
A. (1993). Ceramide-mediated growth inhibition and CAPP are conserved in
Saccharomyces cerevisiae. J. Biol. Chem., 268(5 may), 9255-9261.
Morell, P., & Radin, N. S. (1970). Specificity in ceramide biosynthesis
from
long chain bases and various fatty acyl coenzyme A's by brain
microsomes. J. Biol. Chem., 245(25 jan), 342-350.
Nickels, J. T., & Broach, J. R. (1996, February 15). A ceramide-activated
protein phosphatase mediates ceramide-induced G(1) arrest of
Saccharomyces cerevisiae. Genes & Development, 10(4), 382-394.
Singh, I., & Kishimoto, Y. (1978). A novel synthesis of ceramide from
lignoceric acid and sphingosine by rat barin preparation; the amide
formation requires a pyridine nucleotide. B.B.R.C, 82(29 jun), 1287-
1293.
Thorpe, S. R., & Sweeley, C. C. (1967). Chemistry and metabolism of
sphingolipids. On the biosynthesis of phytosphingosine by yeast.
Biochemistry, 6(mar), 887-898?
Sincerely
F.G. Nobrega
Universidade de Sao Paulo
Departamento de Microbiologia - ICB II / USP
Av. Prof. Lineu Prestes, 1374
05508-900 Sao Paulo - SP - Brasil
Voice: (011) 818-7588 Fax: 55-11-818-7354 E-mail: fgdnobre at usp.br
On 1 Oct 1996 LEIBOWITZ at ocelot.rutgers.edu wrote:
> Dear yeasters,
>> The phosphatase inhibitor okadaic acid inhibits some phosphatases extracted from
> from yeast, as it does similar enzymes from "higher" organisms. However, I
> have been unable to find any information on whether okadaic acid will act on
> the enzymes in intact yeast cells, as it does in cultured vertebrate cells.
> I would be grateful to anyone who can provide info. about the in vivo effect
> of this compound on yeast.
>> Sincerely,
> Mike Leibowitz
> UMDNJ-RW Johnson Medical School
>>
