another thing to look at is to make sure that your strain is not Gal1
minus. These strains can not use Galactose as a carbon source; so
these need to be supplemented with Raffinose as a carbon source. But I
doubt that this is the case in your situation because it would have
been pretty obvious if your culture did not grow.
I concur with the previous post of harvesting after a short induction
time 2-5 hrs versus o/n.
Guy Oshiro
EMAIL: Guy.Oshiro at UCHSC.edu
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