In article <324B08BF.6F11 at BIOSCI.MBP.MISSOURI.EDU>,
NOTHWEHR at BIOSCI.MBP.MISSOURI.EDU wrote:
> N-GST-proteinX-C fusion proteins can be easily purified based on the
> affinity of GST to glutathione-agarose. Does anyone know if it is
> possible to construct fusions to the opposite end of GST (i.e.
> N-proteinX-GST-C) and still maintain proper folding of the GST domain?
> If so, does anyone know if vectors have been constructed which make
> this type of fusion convenient to construct and where I could obtain
> such vectors? Thanks.
We've made N-proteinX-GST-C fusion proteins and they bind glutathione
agarose just fine. Our constructs were made by PCR. I have not noticed
any commercial vectors set up to do this.