Hey Yeasties,
I've taken advantage of the His- phenotype of ade3 strains to
transform plasmids into ade2 ade3 strain. If the strain is HIS3 (and all
the other HIS) then a plasmid carrying ADE3 can be transformed into the
strain. Transformants can be found on SD-his medium.
An article by Beth Jones and Gerald Fink in "The Molecular Biology of
the Yeast Saccharomyces: Metabolism and Gene Expression" (1982) says that
this is because ade3 mutants accumulate 5,10-methylene THF, and this
results in inhibition of the histidine biosynthesis pathway.
On 13 Nov 1996, J. David Stepp wrote:
> Daniel Schott mentioned in passing that ade3 strains cannot synthesize
> histidine. We've seen His auxotrophy in ade3 strains but were unaware that it
> was directly related. Can others confirm this phenomenon? Thanks.
>> Dave Stepp
>>>>>
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@..@ Rick Stevens
(\--/) Department of Biochemistry
(.>__<.) Box 357350
^^^ ^^^ University of Washington
'''''''''''''''' Seattle, WA 98195-7350
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