Ken Wolfe wrote:
>> The ZIP1 gene (GenBank accession number L06487) is missing from the
> Saccharomyces cerevisiae genome sequence! There's nothing remotely
> resembling it on any of the 16 sequenced chromosomes. One of the TIGR
> yeast EST sequences (T38124) from strain X2180-1A matches the original
> GenBank entry, so it seems to be a bona fide yeast gene. ZIP1 has been
> characterised in detail (i.e., 3 Cell papers) and encodes a "synaptonemal
> complex protein required for meiotic chromosome synapsis", which doesn't
> sound like something that might be variably present in different strains.
> Any suggestions?
>> Ken Wolfe
> Trinity College Dublin
>> --
> Ken Wolfe
> Department of Genetics
> University of Dublin e-mail: khwolfe at tcd.ie> Trinity College phone: +353-1-608-1253
> Dublin 2, Ireland FAX: +353-1-679-8558
Dir sir,
Well, I think ZIP1 is a bona fide gene in yeast, too. In a plausible
interpretation for missing the ZIP1 gene from the yeast genome
sequence,
when the gene lies on a region bearing a direct-repeated sequence
flankingly on the yeast genome and the gene with the flanking repeats
cloned into a cosmid is in a rec+ strain of E. coli, ZIP1 may be
deleted from the cosmid via homologous recombination pathway of E.
coli.
However, I think that directors of the sequensing project can use any
rec- mutants of E. coli. So it is likely that such deletion may be
caused even in rec- mutants.
Tohru Ikegami
from faryeast at hgc.ims.u-tokyo.ac.jp
