In article <favre-2105961652170001 at 132.206.101.173>,
favre at medcor.mcgill.ca (Daniel Favre) wrote:
> Dear bionet users,
>> I would like to ask you, whether you have indications where to find the
> full sequence of plasmid pEG202? The latter plasmid is employed in the
> two-hybrid system.
Erica Golemis did some mapping of the plasmid that goes into more details
than map available in CPMB. I'm not sure anybody sequenced the entire
plasmid - it's too bloody long. You can ask Erica but I doubt that the
entire sequence is known. Personally for sequencing primers I used LexA
sequence from Genbank - both primers designed this way work fine. I also
sequenced through the polylinker of pEG202 to get primer for sequencing
the distal end of the fusion =towards the lexA. That's all sequence I've
got.
Regards,
V. Svetlov
--
Of what use is a philosopher who does not hurt anybody's feelings?
Diogenes
