SUC2 [ZIP1 / errors in the genome sequence]

[Mike Cherry]

In article <960521111455.2060e024 at mips.embnet.org>,
 <MEWES at MIPS17.dnet.mips.biochem.mpg.de> wrote:
>...
>Also SUC2 is not found on the genome but this fact can be explained by
>strain variations.
>...

SUC2 is in the genomic sequence.  It is ORF YIL162W.  This was
included in the original submission from Barrell's group for this
region in December of 1994, see SC9402 (accession number Z46921).  The
sequence from Barrell matches the original sequence of SUC2 submitted
by:

     Carlson,M., Taussig,R., Kustu,S. and Botstein,D. (1983) The
      secreted form of invertase in Saccharomyces cerevisiae is
      synthesized from mRNA encoding a signal sequence
      Mol. Cell. Biol. 3 (3), 439-447

That original sequence is SCINVE (Accession Number V01311).

URLs on this topic:

SUC2 Gene:
http://genome-www.stanford.edu/cgi-bin/dbrun/SacchDB?find+Locus+suc2

Original SUC2 Sequence:
http://www3.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=n&form=6&uid=V01311&Dopt=g

Barrell's sequence of clone 9402:
http://www3.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=n&form=6&uid=Z46921&Dopt=g

MIPS chromosome pages:
http://speedy.mips.biochem.mpg.de/htbin/search_contig_by_pos/NINE?start=0&end=50000

Mike

J. Michael Cherry                       Internet: cherry at genome.stanford.edu
Department of Genetics                  Stanford University School of Medicine
Medical Center, Room M311               Stanford, California  94305-5120
Voice: 415-723-7541                     FAX: 415-723-7016