F. Oulette wrote:
>>but I agree with you that it should be somewhere in the genome
If the genetic data are correct, the gene should be located on the
right arm of chr. IV.
We knew the fact that ZIP1 was not on the sequence released (Francis,
it is easy to test this without GenBank annotation!). One lab is already
looking into the matter. Due to the described effects of the disruption
the fact that we don't find ZIP1 or a closely related gene is puzzling.
Also SUC2 is not found on the genome but this fact can be explained by
strain variations.
The 'updates and corrections to come' are well expected. Detailed
investigations on CHRXI have shown that the expected error rate is
around 3 errors/ 10kBases (with a majority of frame-shifts). We can
expect 3000 errors in the sequence. However, if one compares the results
of the systematic sequencing effort to the earlier published sequences,
the error rate is significantly lower (strain differences
may be responsible for some of the differences, but the 'old' sequences
show at least 2 times more errors). Unfortunatly we have to live with
unreliable information in the nucleic acid databases. Despite the 99.7%
of bases that are correct, we cannot distinguish the bad from the good.
Only independent sequencing will significantly lower the error rate. But
this is to expensive for the moment. Of course, major mistakes as lost
fragments, misassembly etc. are different events than individual sequencing
errors. The piece of DNA we are looking for in the case of ZIP1 is at
least 3kBases long.
H. Werner Mewes
MPI f. Biochemie
MIPS
82152 Martinsried
e-mail mewes at mips.embnet.org
mips yeast resources: http://www.mips.biochem.mpg.de/mips/yeast
