In article <3190A136.F0C at infobiogen.fr>, Guillaume Stahl
<stahl at infobiogen.fr> wrote:
> Hi yeastnetters,
> I had some troubles recently to grow new cultures (cerevisiae) from
> old (about one month) liquid cultures conserved at 4C. Someone told
> me that cerevisiae would die rapidly at 4C (on plates or in liquid)
> when they contain a plasmid !???
> Does anyone out there know this story; any informations about this ?
> Any solution ?
>> Thanks
> Guillaume Stahl, France
I haven't had any trouble growing cultures from stock plates kept at 4C for
one month, from yeast with or without plasmids. My experience is mostly
with bacteria, but in general you should always inoculate new cultures with
a single colony picked from an agar plate. A liquid culture kept in the
cold for any length of time may no longer be a clonal population.
--
Deborah Britt, Ph.D.
Medical Oncology
RI Hospital/Brown University
