-- [ From: Yang, Doo Suck * EMC.Ver #2.5.02 ] --
I have some agar plates steaked with recombinant S. cerevisiae.
I have no experience that S. cerevisiae die rapidly at 4°. Usually
microorganisms, including yeasts,
do not die at low temperature. But just stay in dormant phase.
By the way I observed that recombinant plasmid can be cured(?) - i'm not
sure its really cured. anyway it
does'nt work if agar plates kept at 4°C for a long time (above one month)
Yang, Doo Suck
Reserach Scientist
Hanhyo insitutes of Technology
http://www.donald.hanhyo.co.kr/user/microbe
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Hi yeastnetters,
I had some troubles recently to grow new cultures (cerevisiae) from
old (about one month) liquid cultures conserved at 4°C. Someone told
me that cerevisiae would die rapidly at 4°C (on plates or in liquid)
when they contain a plasmid !???
Does anyone out there know this story; any informations about this ?
Any solution ?
Thanks
Guillaume Stahl, France
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Hanhyo Institutes of Tehchnology
Fermentation Lab. Research Scientist
Yang, Doo Suck
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