In article <Pine.ULT.3.91.960506124735.464E-100000 at raphael.acpub.duke.edu>, Namjin Chung <nc1 at acpub.duke.edu> says:
>>>Dear yeast researchers,
>I would like to know any method of direct PCR with yeast clones which are
>picked directly from agar plates. I mean, without preparing DNA after
>growing cells in liquid culture.
>>I have tried one method found in Trends in Genetics (published a few
>years ago) which is simply to PCR after transferring small portion of
>colonies. I was not sure if it was working properly.
>>I would be happy if you have any comments about this. Thank you very
>much in advance.
>>>*************************************************************************
>* | *
>* Namjin Chung | E-mail: nc1 at acpub.duke.edu *
>* Duke University Medical Center | chung006 at mc.duke.edu *
>* Box 3345 | Voice: +1 (919) 684-2363 *
>* Durham, NC 27710 | Fax: +1 (919) 681-8253 *
>* | *
>*************************************************************************
>PCR is working well directly from live unicellular algae using primers on rDNA
(many copies) and the classical hot start.It has been confirmed after sequencing the pcr product.
I think that the common protocol used with E. coli
will work too.
