Namjin Chung wrote:
>> Dear Yeast Researchers,
> Since yeast cells can change their mating types between a and alpha by
> HO, I wonder how we can specify a genotype as MAT a or alpha because,
> for example, cells could have a type after divisions even though you
> started with alpha type. I learned that this switching happens more than
> 50%. Please get me out of this sea of ignorance. Thanks a lot.
>> *************************************************************************
> * | *
> * Namjin Chung | E-mail: nc1 at acpub.duke.edu *
> * Duke University Medical Center | chung006 at mc.duke.edu *
> * Box 3345 | Voice: +1 (919) 684-2363 *
> * Durham, NC 27710 | Fax: +1 (919) 681-8253 *
> * | *
> *************************************************************************
Dear Namjin,
Most lab strains have a mutant ho gene, which codes for an endonuclease essential
to the mating type switching process. If the HO endonuclease is not produced,
mating type switching will not occur. If you would like to know more about this
subject, H. Meiron et al. (1995) Current Genetics, volume 28, 367-373 covers this
area.
Yours,
Gregory Tomlin
*********************
Dept. of Biochemistry,
UMIST, P.O.Box 88,
Manchester, M60 1QD,
U.K.
