Namjin Chung wrote:
>> Dear Yeast Researchers,
> Many may know of color sectoring assay using ade2 ade3 strain and
> ADE3-containing vector. I would like to use this assay for my research,
> and I have read some articles where this methodology were exploited.
> However, I wonder if there are more comprehensive references which
> cover from start to end in detail. Thank you all in advance for any
> comments.
>> *************************************************************************
> * | *
> * Namjin Chung | E-mail: nc1 at acpub.duke.edu *
> * Duke University Medical Center | chung006 at mc.duke.edu *
> * Box 3345 | Voice: +1 (919) 684-2363 *
> * Durham, NC 27710 | Fax: +1 (919) 681-8253 *
> * | *
> *************************************************************************
Dear Namjin,
Greetings again! I presume that you are thinking of a synthetic lethal screen.
A paper that describes this procedure in quite good detail is A.Bender and
J.R.Prigle (1991) Molecular and Cellular Biology, volume 11, 1295-1305. There
are other methods of carrying out a synthetic lethal screen , details of which
I'll send on to you if you are interested.
Yours,
Gregory Tomlin
***********************
Dept. of Biochemistry,
UMIST, P.O.Box 88,
Manchester, M60 1QD,
U.K.
