Direct PCR of yeast clones picked from agar plate?

[Steven Goldberg]

In article
<Pine.ULT.3.91.960506124735.464E-100000 at raphael.acpub.duke.edu>, Namjin 
Chung <nc1 at acpub.duke.edu> wrote:

> Dear yeast researchers,
> I would like to know any method of direct PCR with yeast clones which are 
> picked directly from agar plates.  I mean, without preparing DNA after 
> growing cells in liquid culture.  
> 


I use a modification of the technique in BioTechniques 19:744-748 (1995). 
I swirl a small amount of a yeast colony in 2 ul of 3 mg/ml Zymolase and
incubate at 30 deg. C for at least 15 min, then add this to my PCR
reaction.  I have had excellent results for both S. cerevisiae and P.
pastoris.

Good luck.

Steve