Dear yeast researchers,
I would like to know any method of direct PCR with yeast clones which are
picked directly from agar plates. I mean, without preparing DNA after
growing cells in liquid culture.
I have tried one method found in Trends in Genetics (published a few
years ago) which is simply to PCR after transferring small portion of
colonies. I was not sure if it was working properly.
I would be happy if you have any comments about this. Thank you very
much in advance.
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* Namjin Chung | E-mail: nc1 at acpub.duke.edu *
* Duke University Medical Center | chung006 at mc.duke.edu *
* Box 3345 | Voice: +1 (919) 684-2363 *
* Durham, NC 27710 | Fax: +1 (919) 681-8253 *
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