In article <4m82as$7dd at sol.sun.csd.unb.ca>, sgriffit at rpc.unb.ca (Steve
Griffiths) wrote:
>> I,m trying my darndest to find some hypervariable regions of
> S.cerevisiae strains that are used in the production of lagers.Unlike the
> ale strains, the lager yeasts have only been used for about 150 years.
> All lager strains can be traced to Tubourg or Carlsberg strains.
> There are obvious differences though in overall taste and flavour from
> different strains.
> I am looking at PCR based methods to identify different production
> strains. So far I have tried all manner of single primer RAPD variants as
> well as some degenerate sequences based on the Delta5 and sigma
> sequences. The only success I have had so far is with one of the Delta
> primers on an SSCP format gel.
> Are there any highly variable regions in any of the yeast
> chromosomes that could be used to detect strain drift or stable
> phenotypes?
> TIA STEVEg
Your best bet, in my opinion, would be to look at sub-telomeric regions,
the junkyards of the yeast genome. Ed Louis (elouis at molbiol.ox.ac.uk)
would be an excellent person to ask about this.
Good luck,
--
Michael Lichten
lichten at helix.nih.gov
