I,m trying my darndest to find some hypervariable regions of
S.cerevisiae strains that are used in the production of lagers.Unlike the
ale strains, the lager yeasts have only been used for about 150 years.
All lager strains can be traced to Tubourg or Carlsberg strains.
There are obvious differences though in overall taste and flavour from
different strains.
I am looking at PCR based methods to identify different production
strains. So far I have tried all manner of single primer RAPD variants as
well as some degenerate sequences based on the Delta5 and sigma
sequences. The only success I have had so far is with one of the Delta
primers on an SSCP format gel.
Are there any highly variable regions in any of the yeast
chromosomes that could be used to detect strain drift or stable
phenotypes?
TIA STEVEg
