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trp1-901

Tom Menees tmmenees at CCTR.UMKC.EDU
Wed Mar 27 16:44:23 EST 1996


>Does anybody know what the exact mutation/deletion is in case of trp1-901.
>Trp1-901 is the genotype of many yeast two hybrid strains (such as Y190
>and CG1945).
>
>Thanks,

Magda:

trp1-901 has a 1.4 kbp EcoRI fragment deleted from the TRP1 region.  It
turns out that this deletion also deletes UASgal#1 and UASgal#2 from the
upstream region of the GAL3 gene, which is adjacent to TRP1.  According to
Torchia and Hopper (Mol. Cell. Biol. 8: 3439-3447 (1988)) deletion of
UASgal#1 results in a gal3 mutant phenotype (equivalent to a gal3 null
phenotype).  gal3 mutants exhibit a long term adaptation phenotype which
means they require a lot longer time to fully induce expression of
galactose-regulated genes.  This could be a problem depending on your
experimental needs.  The Torchia and Hopper paper also has a rough map of
the GAL3-TRP1 region.  I hope this information is helpful.


Tom Menees
University of Missouri-Kansas City
Division of Cell Biology and Biophysics
2411 Holmes Street
Kansas City, MO 64108
tel (816)-235-1849
fax (816)-235-5218
email tmmenees at cctr.umkc.edu





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