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expression vector

killer yeast vvsvetlov at utmem1.utmem.edu
Thu Mar 21 08:27:29 EST 1996


In article <01I2K4VZ3YAM9358X5 at CCIT.ARIZONA.EDU>, TSUZUKI at CCIT.ARIZONA.EDU
("T.SUZUKI") wrote:

> Dear All,
> I am a graduate student trying to express a plant small heat shock protein
> in yeast.  I am looking for an expression vector with the following
> properties:
>         1.  tryptophan marker
>         2.  yeast terminator/poly A site
>         3.  Good multiple cloning site for my gene (EcoRI=ideal)
>         4.  Nice strong promoter

It looks like a good candidate for that may be p424GAL1 - 1 out of very
impressive series of bloody 64 (!) plasmids constructed by Mumberg, Muller
and Funk using 4 selectable markers and inducible and constitutive yeast
promoters. You can find the description of the GAL series in NAR, 1994,
22, 5767-5768. Lots of cloning sites, solid German import, works like BMW.
Check with them. We have the stock of all of them (danke!), but  will not
distribute without expressed permission from the authors. 
Regards, 
V. Svetlov

-- 
"EVOLUTION TO A BIOLOGIST IS WHAT SEX IS TO A NORMAL PERSON"
 Dr. S. Carroll



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