In article <314D186D.34DE at hgc.ims.u-tokup.ac.jp>, Tohru Ikegami
<faryeast at hgc.ims.u-tokup.ac.jp> wrote:
LexA or Gal4?
LexA as a bact repressor does not appear to have much of the effect on
yeast when overexpressed alone or as a fusion (unless fusion is toxic by
itself - e.g. strong activator). Gal4 on the other hand has a number of
sites in the yeast genome and at least some of them are functional so that
cell physiology might be affected by the fusion or proteins interacting
with it. Gal11p is known to associate with Gal4p Zn finger (obviously
present in Gal4BD) which may or may not occur in fusion but a possibility
exist that such interaction - and Gal11p is a big protein - might obscure
certain types of interactions esp. with a small fusion. It appears that
the variety of reporters for LexA based fusions in terms of number of
binding sites is greater.
"EVOLUTION TO A BIOLOGIST IS WHAT SEX IS TO A NORMAL PERSON"
Dr. S. Carroll