I have a few questions about two-hybrid system in Saccharomyses
I'd like to find Baker's yeast proteins that interact with our yeast
protein fused LexA or GAL4(1-147) as the "bait" for yeast two-hybrid
Is there any advantages or disadvantages in each case of LexA or GAL4
using for two-hybrid system in general?
How about in the case of using hi-expression vector ex.
Thanks a lot for any advice.
Mol. Biol. Inst. Med.Sci.
Univ. Tokyo, JAPAN
faryeast at ims.u-tokyo.ac.jp