In article <4ibtbl$8qg at masala.cc.uh.edu>, yoas at cfox.bchs.uh.edu (Karen
Yoas) wrote:
> Does anyone have advice or tips for using the micromanipulator for
> picking up
> asci? (Besides lots and lots of patience...)
Make a new needle. Make sure that your plates are dry (left unbagged >2
days after pouring). Think only clean thoughts.
> How do you prevent the
> cells from clumping when you put them on the plate? I have tried
> vortexing and pipetting up and down, but I still have lots of cells
> clumped together.
One way to prevent cell clumping is to lightly sonicate. We generally set
our sonicator on 0 (obviously not really 0) and sonicate for a few
seconds. I have also found that the inclusion of 0.1% TWEEN-80 helps.
--
Michael Lichten
lichten at helix.nih.gov
