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pombe - isolating tetrads

Karen Yoas yoas at cfox.bchs.uh.edu
Fri Mar 15 09:03:33 EST 1996

Does anyone have advice or tips for using the micromanipulator for
picking up
asci?  (Besides lots and lots of patience...)  How do you prevent the
cells from clumping when you put them on the plate?  I have tried
vortexing and pipetting up and down, but I still have lots of cells
clumped together.

Karen Yoas
Biological & Biochemical Sciences
University of Houston
Houston, TX 77204-5934

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