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yeast genotype

Dan Baggott dbaggott at reed.edu
Thu Mar 14 12:52:29 EST 1996

namy <namy at lovelace.infobiogen.fr> wrote:
>Does anybody know what is the mutation and the phenotype "can1-100"? 
>(my strain is HFY1200)
>Thank you for your answer.

CAN1 encodes an arginine permease which when absent prevents cells from
uptaking the arginine analog canavanine.  CAN1 cells grown in media
lacking arginine and containing canavanine (60 microg/ml) incorporate
the analog into proteins and die.  can1 mutants can't take-up canavanine
and are therefore resistant.  I don't know what the actual can1-100
mutation is.

Dan Baggott
Reed College Biology Dept.
Portland, OR

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