poonso at NWG.NECTEC.OR.TH (Poonsook Orprayoon) writes:
>I wonder if anyone know what is STE13 gene product in yeast , Pichia in
>this case? As I understand, it is a protease that cut Glu-Ala repeat??
>If I have Glu-Ala in other region of the protein, will STE13 gene product
>cut it as well?
>Also, I am looking for protease negative strain of Pichia pastoris. Let
>me know if anyone have. Thanks.
In cerevisiae, the product of the STE13 gene is a type IV dipeptidyl
aminopeptidase that removes X-Ala dipeptides from the amino-termini of
alpha factor processing intermediates. Like other type IV DPAPases it
can also remove X-Pro dipeptides from amino-termini and it actually
removes a couple of these dipeptides from the mature amino-terminus of
Kex2 protease. Because it is an aminopeptidase, no it will not remove
internal Glu-Ala sequences.
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