On 7 Mar 1996 LEIBOWITZ at ocelot.rutgers.edu wrote:
> As I already emailed Rich, I have had no problem mating yeasts in liquid,
> although I have not done so 96 wells at a time. Perhaps the source of the
> problem you describe might be that as assayed on plates, etc., alpha cells
> produce much more mating factor activity than do a cells, i.e., it is easy to
> demonstrate a shmooing in response to alpha cells but harder to demonstrate
> alpha shmooing in response to a cells. I don't know if this difference (seen
> obviously in wild-type cells, since mutants can be made to do anything)
> reflects differences in amount of mating factor produced or mating factor
> specific activity differences. I'm curious, do you find you need more a's than
> alpha's, as my explanation would imply?
>> Sincerely,
> Mike Leibowitz
> UMDNJ-RW Johnson Medical School
>thanks for your reply. My experiment requires that all my a cells mate;
therefore i have been using an excess of alphas.
my a strain has different nutritional requirements from my alpha strain,
so when i plate out my culture all the haploid alphas die and hopefully
all that survive are diploids. alas, i seem to still get a lot of haploid
a cells.
regards,
romola
Romola Davenport
Dept of Plant Sciences
Downing St Cambridge
(01223) 330 210 (lab)
(01223) 311 408 (home)
