In article <4hkvcr$dvb at ussun2n.glaxo.com>, Rich Buckholz <rgb12955 at glaxo.com> writes:
> Does anyone have experience with mating S. cerevisiae in liquid culture
> vs on plates? Specifically what I am trying to do is mate lots of a's
> with lots of alpha's and want to avoid using lots of plates (even cross
> streaking is too much). So, I want to try it out with 96-well plates:
> haploids pregrown in wells in selective media, then multichannel
> pipetted to YPD for growth/mating. Then I can frog them to selective
> plates or media as needed.
>In our lab we have good experience with matings in
liquid culture, the efficiency is even higher in most cases.
You can do it with some cells taken directly from a plate,
suspended in 1 ml YPD and incubated for at least 3 hours
on a turning wheel. Our wildtype strain RS453 is known to
be a good mater. Problems could arise on microtiter wells
because of insufficient mixing.
Institut fuer Biochemie I