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Rich Buckholz rgb12955 at glaxo.com
Wed Mar 6 16:17:15 EST 1996

Does anyone have experience with mating S. cerevisiae in liquid culture 
vs on plates?  Specifically what I am trying to do is mate lots of a's 
with lots of alpha's and want to avoid using lots of plates (even cross 
streaking is too much).  So, I want to try it out with 96-well plates:  
haploids pregrown in wells in selective media, then multichannel 
pipetted to YPD for growth/mating.  Then I can frog them to selective 
plates or media as needed.


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