Does anyone have experience with mating S. cerevisiae in liquid culture
vs on plates? Specifically what I am trying to do is mate lots of a's
with lots of alpha's and want to avoid using lots of plates (even cross
streaking is too much). So, I want to try it out with 96-well plates:
haploids pregrown in wells in selective media, then multichannel
pipetted to YPD for growth/mating. Then I can frog them to selective
plates or media as needed.
Thanks,
Rich
