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2hydri

Didier Fesquet fesquet at KAA.CRBM.CNRS-MOP.FR
Fri Mar 1 07:57:08 EST 1996


hi,

during a two hubrid screen, upon recovery and Ecoli transformation of
plasmid from his+lacwhen we transform back yeast to check for specific interaction. the plasmid display still HIS+ phenotype but not lac+.
does anybody encounter this problem. how can we explain this?
thanks



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