To whoever can help me,
I have recently obtained the yeast expression vector YEpFLAG-1 (from Kodak) and cloned my gene of
interest into it and then electroporated this into the host strain BJ 3505. When I checked my colonies
a few days later (on a SCM -trp plate), I noticed 2 types of colonies on the plate;
(1) rather large, cream colored, broad colonies and;
(2) smaller, white-colored, well defined colonies.
When I grew these colonies in some SCM -trp media, the cream colonies grew quite well, while the white
colonies took an extra day or two to grow up (yeast containing no vector didn't grow at all-neg.
control). When grown in a complete media containg peptone, glucose, glycerol, etc..., the cream
colonies only grew to a low cell density, while the white colonies grew to a higher density.
If anyone out there has used the YEpFLAG1 vector or has any ideas what is going wrong(?!?!) with this,
could you please contact me via e-mail on; small_group.pathology at muwaye.unimelb.edu.au
Thanking you for your time and assistence,
Dept. of Pathology,
University of Melbourne,