I need to find protocols for Northern and ribosomal RNA electrophoresis
courrently working with yeast and I have tecnicals troubles.
I have run electrophoresis 5, 10, 15 ug of rRNA was extracted with:
a) acidified phenol, heating to 65§ C.
b) phenol-clorophormo- isoamilic and glass beads(bortex).
and cuantified by OD at 260 nm.
I heve also stain the agarose gels with etidium bromide and I have not
seen the bands 25S and 18S rRNA.
All my glassware has been trated with DEPC.
I shall be gratefull for any help.
* Isidro Contreras Morales * Email: a73055 at uachih.uachnet.mx
* Division de Estudioos de Posgrado
* Facultad de Ciencias Quimicas * Tel. (14) 13-18-28 Ext.117
* Universidad Autonoma de Chihuahua * Fax. (14) 14-44-92
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