microbe at DONALD.HANHYO.CO.KR ("Yang, Doo Suck") wrote:
>-- [ From: Yang, Doo Suck * EMC.Ver #2.5.02 ] --
>>I was given a yeast host strain.
>It named as below.
>S. cerevisiae 2805
>a, pep4::HIS3, pro1-delta, can 1, Gal 2, his3delta, uar3-52
>>I think that those notations are decribing genetic structures or status.
>But I have no idea what they mean exactly.
>>Explain the terms for me, please.
>>You will probably get a number of answers, but what the heck. The pep4::HIS3 means
that the HIS3 gene has been inserted into the coding region of PEP4, creating a
"disruption" mutation. Such a mutation eliminates several of the major proteases.
pro1-delta refers to a deletion in the PRO1 gene, probably created by recombinant
methods. This gene product is required in proline synthesis.
can1 is a mutation in the arginine permease gene causing canavanine resistance
Gal2 (should be gal2) is a mutation in a gene of galactose utilization (the
permease)
his3delta is a mutation in HIS3 which removes the entire coding region and causes
histidine auxotrophy
ura3-52 is a mutation in URA3 created by a Ty transposon insertion. It causes
uracil auxotrophy and reverts at very low frequency.
This strain is mating type a.
Good luck.
Beth Montelone
Kansas State University
bethmont at ksu.ksu.edu
