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Would you explain geneic notation like pep4::HIS3 etc.

Beth A. Montelone bethmont at ksu.ksu.edu
Tue Jun 11 12:47:43 EST 1996


microbe at DONALD.HANHYO.CO.KR ("Yang, Doo Suck") wrote:
>-- [ From: Yang, Doo Suck * EMC.Ver #2.5.02 ] --
>
>I was given a yeast host strain.
>It named as below.
>S. cerevisiae 2805
>a, pep4::HIS3, pro1-delta, can 1, Gal 2, his3delta, uar3-52
>
>I think that those notations are decribing genetic structures or status.
>But I have no idea what they mean exactly.
>
>Explain the terms for me, please.
>
>
You will probably get a number of answers, but what the heck.  The pep4::HIS3 means 
that the HIS3 gene has been inserted into the coding region of PEP4, creating a 
"disruption" mutation. Such a mutation eliminates several of the major proteases. 

pro1-delta refers to a deletion in the PRO1 gene, probably created by recombinant 
methods.  This gene product is required in proline synthesis.

can1 is a mutation in the arginine permease gene causing canavanine resistance

Gal2 (should be gal2) is a mutation in a gene of galactose utilization (the 
permease)

his3delta is a mutation in HIS3 which removes the entire coding region and causes 
histidine auxotrophy

ura3-52 is a mutation in URA3 created by a Ty transposon insertion.  It causes 
uracil auxotrophy and reverts at very low frequency.

This strain is mating type a.

Good luck.

Beth Montelone
Kansas State University
bethmont at ksu.ksu.edu




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