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ts and cs at the same time?

Bawagan, Hinayana L bawagan at OCELOT.RUTGERS.EDU
Tue Jun 11 14:34:50 EST 1996


I have made a number of ts mutations in my favorite gene (I don't know what it
does, I only know it's essential).  I did the usual plasmid shuffle and defined
my permissive temperature as 30¼ C.  Now I have mutants that don't grow at 37¼
C. and it turns out that most of them are also VERY unhappy at room temp too. 
This is true on plates as well as liquid.  I wasn't expecting this.  Even at the
permissive temp the cells don't look happy under the microscope (big, swollen
cells), but they definitely grow (about 2 hour doubling time).  Meanwhile, our
WT is very happy at all three temps.

Has anyone seen this or have any words of inspiration?

Yen Bawagan
bawagan at mbcl.rutgers.edu

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