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Northerns

Marguerite Evans p2158740 at ACSUSUN.ACSU.UNSW.EDU.AU
Thu Jun 6 19:22:32 EST 1996


Hi everyone,

We have had quite a few letters regarding the protocol members in my lab 
now have working for Northerns.  It is in the manual "Methods in Yeast 
Genetics" A Cold Spring Harbour Laboratory Course Manual.  1994 edition 
by C. Kaiser, S. Michaelis and A. Mitchell.  It is quite a long protocol 
but I am assured it is worth the extra effort.  With regards to the 
herring sperm DNA, it was too big (>300bp) so get some that you can shear 
to below this (we now use some grungy looking calf thymus DNA and it 
seems to work).  I have yet to personally get this going, but I am 
confident having observed the nice results of others in my lab.

Good luck!
Marguerite.


Marguerite Evans
School of Biochemistry and Molecular Genetics
UNSW  Sydney  2052
NSW
Australia

voice: (+61)(2)385 2030
fax:   (+61)(2)313 6271
email: p2158740 at acsusun.acsu.unsw.edu.au




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