I've integrated recently a fusion protein with wt GFP en Cterm in the
cerevisiae genome. After YPD culture (+/- 1 OD), directly under scope,
using fluoresceine filters, I've not been able to see anything (except
some cells, strange shape, greennish/yellowish). Same result with same
culture O.N. in the fridge, and same result with... negative control.
My question is simple : what are the condition (media, OD, wash, fridge,
filters,...) you used if you ever saw GFP in yeast. Do you think a
single copy of 'medium expressed' GFP is sufficient. Should I try it in
a FACS ? Do you have good conditions/suggestions for the last point ?
Thanks in advance
Guillaume Stahl, Orsay, France