We are trying to rescue Leu2 based plasmids from yeast (also
containing TRP based plasmids). It doesn't look like the M9 media
supplemented with proline, thiamine, and amp is keeping the TRP based
plasmids from recueing. As a control, I transformed pGEMt, and it
survived on these plates as well. Has anyone else out there had this
problem? I have tried another aliquot of HB101 cells and am ordering
more from promega.
Thanks in advance,