Dear colleagues:
For the purpose of studying the sequence elements of a yeast promoter using
the beta-galactosidase transcriptional fusion one should clone just the
intergenic DNA sequence between your favorite gene and the end of the
upstream gene or the start of a divergent upstream reading frame.
Is that correct? Is it possible that activating or repressing elements
for a given promoter are located inside a neighbors reading frame?
Thanks for your comments!
Francisco
F.G. Nobrega
Universidade de Sao Paulo
Departamento de Microbiologia - ICB II / USP
Av. Prof. Lineu Prestes, 1374
05508-900 Sao Paulo - SP - Brasil
Voice: (011) 818-7588 Fax: 55-11-818-7354 E-mail: fgdnobre at usp.br
