For the purpose of studying the sequence elements of a yeast promoter using
the beta-galactosidase transcriptional fusion one should clone just the
intergenic DNA sequence between your favorite gene and the end of the
upstream gene or the start of a divergent upstream reading frame.
Is that correct? Is it possible that activating or repressing elements
for a given promoter are located inside a neighbors reading frame?
Thanks for your comments!
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