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Francisco G da Nobrega ib - bio 7588 fgdnobre at usp.br
Wed Jun 5 16:58:14 EST 1996

Dear colleagues:

For the purpose of studying the sequence elements of a yeast promoter using
the beta-galactosidase transcriptional fusion one should clone just the
intergenic DNA sequence between your favorite gene and the end of the 
upstream gene or the start of a divergent upstream reading frame. 
Is that correct? Is it possible that activating or repressing elements
for a given promoter are located inside a neighbors reading frame?

Thanks for your comments!


F.G. Nobrega
Universidade de Sao Paulo
Departamento de Microbiologia - ICB II / USP
Av. Prof. Lineu Prestes, 1374
05508-900   Sao Paulo  -  SP  - Brasil
Voice: (011) 818-7588    Fax: 55-11-818-7354    E-mail:  fgdnobre at usp.br

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