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His-tagged pichia vectors

Mikkel Holmen Andersen mha at biobase.dk
Tue Jul 30 09:42:06 EST 1996


hi

Does anyone know if a expression vector for the Pichia pastoris
expression system is available. We are interested in an expression vector
coding for a N-terminal His-tag followed by a protease site and then the
protein of interest (HIS-cleave site-protein). This would ease
purification somewhat.

We are aware of the Invitrogen pPICZ A,B,C vectors, but these do not
leave any possibility to remove the His-tag after purification.


Thank you;

M. H. Andersen

Protein Chemistry Laboratory
University of Aarhus
Denmark



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