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expression vectors

Scott Kaufmann shkaufma at email.unc.edu
Tue Jul 30 22:57:36 EST 1996

Hi all.  I am trying to create yeast expression vectors using promoters 
(both ADH and GAL1) from various yeast two-hybrid vectors and some RS 
shuttle vectors that we have in our lab.  I have noticed that these 
vectors use various fragments of the promoter and was wondering if 
anyone had some advice as to the best length of each promoter for the 
highest expression.

By the way, if someone already has some expression vectors that they are 
willing to share, it would be greatly appreciated.


Scott Kaufmann
Universtiy of North Carolina
  at Chapel Hill

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