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Yeast Membranes and Cholesterol?

Kevin Morano kmorano at umich.edu
Tue Jul 30 22:18:40 EST 1996


Hi Cara,

   At least S.c. does not have cholesterol, or at least not very much.  In
my previous lab we wanted to use the membrane permeabilizer streptolysin O
(SLO) for semi-in vitro experiments and it binds to cholesterol and forms
pores.  It does not work in S.c..  I believe the main membrane sterol is
ergosterol, which SLO just laughs at.

   I think P.p. might have cholesterol because I seem to recall SLO being
used successfully in Subramani's lab looking at peroxisome import.  I
would start there and also look up references by Daum, an authority on
yeast lipids and membranes.

Kevin Morano
Thiele Lab



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