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Tightly regulated inducible promoter

James R. Bone jrbone at onramp.net
Thu Jul 25 14:06:42 EST 1996


I am looking for a promoter or promoter system that allows high induction
from an inactive (no expression) promoter.  The GAL promoter still has
some expression in glucose.  Is there something similar to the pET vector
system used in bacteria--  induce expression of T7 polymerase that allows
expression from T7 promter of your favorite gene.  Very tight regulation.

Thanks,

Jim Bone



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