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Fission: loss of plasmid

Hoffmacs Hoffmacs at HERMES.BC.EDU
Thu Jul 25 06:40:29 EST 1996

Dear Zhaomin et al.,
Even with ars sequences, we see a "healthy" loss of plasmid in a nonselective
medium.  Plasmid pFL20 has both an ars and a stb sequence.  I believe the
second element is what really boosts the retention of this plasmid (check out
MCB 6: 80-89).  We often want to lose a plasmid to test for plasmid-conferred
phenotypes while screening a library.  Two to three days growth on YEA is
plenty for this purpose.   Hope this helps.  Be sure to check out the
"Pombology: A Fission Expedition" workshop if you are at the meeting in
Madison next month.
Charlie Hoffman
I'd rather be fission.

Hi, all,

I have a quick question for the fissoners. If a plasmid has an ARS from 
S. pombe, what is the chance for the plasmid to be lost after a few 
generations of growth in non-selective media? Good? Bad? One in a 
million, in a thousand, or in a hundred?



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