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yeast tRNA amber suppressor

Ken Howe howe at DARWIN.UCSC.EDU
Wed Jul 24 13:36:45 EST 1996

this is a repeat of a query I posted to methods-and-reagents so please
excuse the redundancy, but I wanted to reach those of you who don't
subscribe that that news group.

I'm looking for a yeast tRNA amber suppressor of any type; although the
amber mutation was originally a glutamine codon, it doesn't really matter
the identity of the replacement amino acid.

I should be able to express the suppressor off a yeast vector for in vivo
experiments.  If anyone has or knows of such a beast, please contact me at
my e-mail: howe at darwin.ucsc.edu.


Ken Howe
Center for the Molecular Biology of RNA
University of California
Santa Cruz, CA  95064

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