Hello yeast newsgroup
I have been looking at protocols for RNA extraction suitable for
S.cervisiae which will give me good undegraded mRNA for Differential
Display. Most do not mention mRNA quality. I am not experienced with
yeast but I think the method needs to be fairly harsh to break down the
cell wall but I am concerned that the mRNA will be damaged by shearing.
My supervisor has suggested an enzyme treatment to soften the cell wall
followed by a detergent type of extraction. If you have any experience
or thoughts on this problem I would be most grateful to hear about them.