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mRNA for Differential Display

Dianne Emslie s9033036 at COUGAR.VUT.EDU.AU
Mon Jul 22 16:48:16 EST 1996

Hello yeast newsgroup
I have been looking at protocols for RNA extraction suitable for 
S.cervisiae which will give me good undegraded mRNA for Differential 
Display. Most do not mention mRNA quality. I am not experienced with 
yeast but I think the method needs to be fairly harsh to break down the 
cell wall but I am concerned that the mRNA will be damaged by shearing. 
My supervisor has suggested an enzyme treatment to soften the cell wall 
followed by a detergent type of extraction.  If you have any experience 
or thoughts on this problem I would be most grateful to hear about them.
Cheers, Dianne  

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