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Spheroplast fusion w/mammalian cells??

Andrei Popov andrei.popov at bbsrc.ac.uk
Wed Jul 17 07:22:31 EST 1996


Andrea Marie Peier wrote:
> 
> Hi,
> 
> I'm trying to find a protocol that describes fusion of yeast speroplasts
> with mammalian cells. Does anyone have such a procedure they could
> e-mail or fax me??
> 
> Thanks,
> Andrea

Hello Andrea,
the ref. you are looking for is:

Pachnis et al. PNAS (1990) 87: 5109-13.

The fusion is not a very simple protocol and may take months
to establish; very much is dependent on the selectable marker.
If you need more details e-mail me.
regards

Andrei



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