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yeast vesicles reconstitution

Jose Maria Ortega Ruiz mi1orruj at lucano.uco.es
Wed Jul 17 05:42:49 EST 1996

I am trying to obtain yeast vesicles trough Sephadex G-50 columne. I 
use octyl-glucoside as detergent ( Venema and Palmgren, 1995)I don t 
obtain well selled vesicles and then I lost the ATPase activity during the process. Can the detergent 
affect the enziymatic activity? How can I solvent that my vesicles don t 
	Thanks in advance!!   
			Flori Aguilera
		Dpto. Microbiology. Faculty of Science
			University of Cordoba
	     	Avd. S. Alberto Magno s/n
			14004 Cordoba

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