<Pine.SOL.3.91.960716142607.28951C-100000 at godzilla4.acpub.duke.edu>,
Namjin Chung <nc1 at acpub.duke.edu> wrote:
->I'm working with an uncharacterized yeast enzyme activity, and it seems
->to have activity around pH 2.5 and 3.0. Currently I'm using Citric Acid
->and Citrate-Phosphate buffers to get a decent result. The activity is,
->however, inconsistent-standard deviation is high from experiment to exp.
->I guess that harsh pH like 2.5 itself might have killed the enzyme
->activity to give varying results, but is that possible? If pH itself
->were the matter here, it should always kill the enzyme, and if there is
->the enzyme activity around this pH, the enzyme should survive at this
->So I suspect the buffers (citrate and citrate-phosphate) may not be good
->at this pH. Is there anyone who had this kind of experience, and/or who
->could recommend a good buffer for this pH range (2.5-3.0). Thank you in
->advance for comments.
I wouldn't blame buffers for that. Citrate-phosphate is exactly what
I'd use for this range. And they are perfectly "good" and stable.